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. 2011 Dec;49(12):4301-3.
doi: 10.1128/JCM.05602-11. Epub 2011 Oct 19.

Sensitive and specific modified Hodge test for KPC and metallo-beta- lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603

Fernando Pasteran  1 , Omar Veliz, Melina Rapoport, Leonor Guerriero, Alejandra Corso
Affiliations

Sensitive and specific modified Hodge test for KPC and metallo-beta- lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603

Fernando Pasteran et al. J Clin Microbiol. 2011 Dec.

Abstract

We evaluated the ability of the modified Hodge test to discriminate between KPC- and metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates and carbapenemase nonproducers. With Escherichia coli ATCC 25922 as the indicator strain, the MHT resulted in low sensitivity, specificity, and repeatability. Replacing the indicator strain with Klebsiella pneumoniae ATCC 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeatability, respectively).

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Figures

Fig. 1.
Fig. 1.
Results of the modified Hodge test for a representative VIM-producing P. aeruginosa isolate. Comparative performance was assessed with E. coli ATCC 25922, P. aeruginosa ATCC 27853, and K. pneumoniae ATCC 700603 as indicator strains. The final interpretation of the MHT is shown below each strain.
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