Review
doi: 10.1002/wrna.112.
Epub 2011 Oct 19.
The mechanism of the second step of pre-mRNA splicing
Affiliations
- PMID: 22012849
- DOI: 10.1002/wrna.112
Item in Clipboard
Review
The mechanism of the second step of pre-mRNA splicing
Wiley Interdiscip Rev RNA.
2012 May-Jun.
Abstract
The molecular mechanisms of the second step of pre-mRNA splicing in yeast and higher eukaryotes are reviewed. The important elements in the pre-mRNA, the participating proteins, and the proposed secondary structures and roles of the snRNAs are described. The sequence of events in the second step is presented, focusing on the actions of the proteins in setting up and facilitating the second reaction. Mechanisms for avoiding errors in splicing are discussed.
Copyright © 2011 John Wiley & Sons, Ltd.
Similar articles
-
Helicases involved in splicing from malaria parasite Plasmodium falciparum.Parasitol Int. 2011 Dec;60(4):335-40. doi: 10.1016/j.parint.2011.09.007. Epub 2011 Oct 1. Parasitol Int. 2011. PMID: 21996352 Review.
-
Secondary structure of the yeast Saccharomyces cerevisiae pre-U3A snoRNA and its implication for splicing efficiency.RNA. 1996 Nov;2(11):1079-93. RNA. 1996. PMID: 8903339 Free PMC article.
-
Yeast Prp2 liberates the 5' splice site and the branch site adenosine for catalysis of pre-mRNA splicing.RNA. 2017 Dec;23(12):1770-1779. doi: 10.1261/rna.063115.117. Epub 2017 Sep 1. RNA. 2017. PMID: 28864812 Free PMC article.
-
Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites.Cell. 2016 Feb 25;164(5):985-98. doi: 10.1016/j.cell.2016.01.025. Cell. 2016. PMID: 26919433 Free PMC article.
-
Pseudouridines in spliceosomal snRNAs.Protein Cell. 2011 Sep;2(9):712-25. doi: 10.1007/s13238-011-1087-1. Epub 2011 Oct 6. Protein Cell. 2011. PMID: 21976061 Free PMC article. Review.
Cited by
-
A Genetic Screen Identifies PRP18a, a Putative Second Step Splicing Factor Important for Alternative Splicing and a Normal Phenotype in Arabidopsis thaliana.G3 (Bethesda). 2018 Mar 28;8(4):1367-1377. doi: 10.1534/g3.118.200022. G3 (Bethesda). 2018. PMID: 29487188 Free PMC article.
-
Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2.Nucleic Acids Res. 2017 Apr 20;45(7):4068-4080. doi: 10.1093/nar/gkw1225. Nucleic Acids Res. 2017. PMID: 27923990 Free PMC article.
-
Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system.RNA. 2013 Jul;19(7):902-15. doi: 10.1261/rna.039024.113. Epub 2013 May 17. RNA. 2013. PMID: 23685439 Free PMC article.
-
A broad analysis of splicing regulation in yeast using a large library of synthetic introns.PLoS Genet. 2021 Sep 27;17(9):e1009805. doi: 10.1371/journal.pgen.1009805. eCollection 2021 Sep. PLoS Genet. 2021. PMID: 34570750 Free PMC article.
-
Prevalent and distinct spliceosomal 3'-end processing mechanisms for fungal telomerase RNA.Nat Commun. 2015 Jan 19;6:6105. doi: 10.1038/ncomms7105. Nat Commun. 2015. PMID: 25598218 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
