Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Dec 2;50(49):11769-72.
doi: 10.1002/anie.201105508. Epub 2011 Oct 19.

Dendrimer-mediated multivalent binding for the enhanced capture of tumor cells

Ja Hye Myung  1 , Khyati A Gajjar, Jelena Saric, David T Eddington, Seungpyo Hong
Affiliations

Dendrimer-mediated multivalent binding for the enhanced capture of tumor cells

Ja Hye Myung et al. Angew Chem Int Ed Engl. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Schematic illustration and fluorescence images of tumor cell capture using aEpCAM on a) dendrimer- and b) linear polymer-immobilized surfaces.
Figure 2
Figure 2
Enhanced cell adhesion and binding stability on the dendirmer-coated surfaces under static conditions. a) The ratios of the numbers of the bound cancer cells on the dendrimer-immobilized surfaces to those on the PEGylated surfaces. Error bars: standard error (n > 3). b) Dissociation kinetics of MDA-MB-361 cells on the dendrimer-immobilized surfaces and the PEGylated surfaces. The dendrimer-immobilized surfaces significantly increase the fraction of remaining cells on the surfaces upon static agitation as compared to the PEGylated surfaces even after reducing the amount of aEpCAM added. Error bars: standard error (n=3). c) Recovery yields of the captured MDA-MB-361 cells using various numbers (10, 20, 200, and 1,000) of the cells spiked with and without HL-60 cells. Significant improvements of the dendrimer surfaces were observed when either 103 of cancer cells were applied or the cells were mixed with HL-60 cells (107 cells per surface). Error bars: standard error (n=3). Asterisks indicate p < 0.05.
Figure 3
Figure 3
Enhanced cell binding stability by combination of multivalent binding and cell rolling under flow. Three substrates, epoxy-functionalized, PEGylated, and dendrimer-immobilized surfaces, treated with aEpCAM alone (a) or with both aEpCAM and E-selectin (b) were compared in terms of capture efficiency. The captured cancer cells on the surfaces were counted after injection of cell suspensions and washing with PBS at a shear stress of 0.8 dyn cm−2, followed by normalization based on the number of each cell line on the epoxy-functionalized surfaces without E-selectin. Up to 7-fold enhancement in the capture efficiency by the dendrimer-immobilized surface was achieved through combination of rolling (E-selectin) and multivalent binding (aEpCAM). Error bars: standard error (n=3).
See this image and copyright information in PMC

References

    1. Lee RT, Lee YC. Glycoconj J. 2000;17:543. - PubMed
    2. Mammen M, Choi SK, Whitesides GM. Angew Chem Int Edit. 1998;37:2755. - PubMed
    3. Mourez M, Kane RS, Mogridge J, Metallo S, Deschatelets P, Sellman BR, Whitesides GM, Collier RJ. Nat Biotechnol. 2001;19:958. - PubMed
    1. Hong S, Leroueil PR, Majoros IJ, Orr BG, Baker JR, Jr, Banaszak Holl MM. Chem Biol. 2007;14:107. - PubMed
    2. Kiessling LL, Pohl NL. Chem Biol. 1996;3:71. - PubMed
    3. Quintana A, Raczka E, Piehler L, Lee I, Myc A, Majoros I, Patri AK, Thomas T, Mule J, Baker JR., Jr Pharm Res. 2002;19:1310. - PubMed
    4. Peer D, Karp JM, Hong S, Farokhzad OC, Margalit R, Langer R. Nat Nanotechnol. 2007;2:751. - PubMed
    5. Weissleder R, Kelly K, Sun EY, Shtatland T, Josephson L. Nat Biotechnol. 2005;23:1418. - PubMed
    1. Christensen T, Gooden DM, Kung JE, Toone EJ. J Am Chem Soc. 2003;125:7357. - PubMed
    2. Gestwicki JE, Cairo CW, Mann DA, Owen RM, Kiessling LL. Anal Biochem. 2002;305:149. - PubMed
    3. Kitov PI, Bundle DR. J Am Chem Soc. 2003;125:16271. - PubMed
    1. Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M. Nature. 2007;450:1235. - PMC - PubMed
    2. Wang S, Wang H, Jiao J, Chen KJ, Owens GE, Kamei K, Sun J, Sherman DJ, Behrenbruch CP, Wu H, Tseng HR. Angew Chem Int Ed Engl. 2009;48:8970. - PMC - PubMed
    1. Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, Floyd FP, Jr, Gilman AJ, Lord JB, Winokur D, Springer S, Irimia D, Nagrath S, Sequist LV, Lee RJ, Isselbacher KJ, Maheswaran S, Haber DA, Toner M. Proc Natl Acad Sci USA. 2010;107:18392. - PMC - PubMed
    2. Wang S, Liu K, Liu J, Yu ZT, Xu X, Zhao L, Lee T, Lee EK, Reiss J, Lee YK, Chung LW, Huang J, Rettig M, Seligson D, Duraiswamy KN, Shen CK, Tseng HR. Angew Chem Int Ed Engl. 2011;50:3084. - PMC - PubMed

Publication types