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. 2011:2011:104284.
doi: 10.1155/2011/104284. Epub 2011 Oct 13.

Incidence of malaria in the interior division of sabah, malaysian borneo, based on nested PCR

Wan Fen Joveen-Neoh  1 , Ka Lung Chong, Clemente Michael Vui Ling Wong, Tiek Ying Lau
Affiliations

Incidence of malaria in the interior division of sabah, malaysian borneo, based on nested PCR

Wan Fen Joveen-Neoh et al. J Parasitol Res. 2011.

Abstract

Introduction. Malaria is currently one of the most prevalent parasite-transmitted diseases caused by parasites of the genus Plasmodium. Misidentification of human malaria parasites especially P. knowlesi based on microscopic examination is very common. The objectives of this paper were to accurately identify the incidence of human malaria parasites in the interior division of Sabah, Malaysian Borneo, based on small subunit ribosomal RNA (ssrRNA) and to determine the misidentification rate in human malaria parasites. Methods. Nested PCR was used to detect the presence of human malaria parasites. A total of 243 blood spot samples from patients who had requested for blood film for malaria parasite (BFMP) analyses were used in this study. Results. Nested PCR findings showed that there was no P. malariae infection while the highest prevalent malaria parasite was P. knowlesi, followed by P. vivax, P. falciparum, and mixed infection. Only 69.5% of the 243 samples giving consistent nested PCR and microscopic results. Conclusion. The preliminary findings from molecular detection of malaria showed that P. knowlesi was the most prevalent Plasmodium species in the interior division of Sabah. The findings from this paper may provide a clearer picture on the actual transmission of different Plasmodium species in this region.

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Figures

Figure 1
Figure 1
Map of the Sabah state indicating the location of the study sites in this study.
Figure 2
Figure 2
Distribution of Plasmodium species according to study sites based on microscopic findings. The numbers in the chart indicated the number of cases of Plasmodium species in each study site.
Figure 3
Figure 3
Authentication of Plasmodium species in the interior division of Sabah. The numbers in the pie chart denoted number of positive cases.
Figure 4
Figure 4
Distribution of human malaria parasites in the interior division of Sabah based on nested PCR. Numbers in the chart denoted number of cases detected.
See this image and copyright information in PMC

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