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. 2011:2011:369484.
doi: 10.1155/2011/369484. Epub 2011 Oct 15.

Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Rosaria Medda  1 , Francesca Pintus, Delia Spanò, Giovanni Floris
Affiliations

Bioseparation of Four Proteins from Euphorbia characias Latex: Amine Oxidase, Peroxidase, Nucleotide Pyrophosphatase/Phosphodiesterase, and Purple Acid Phosphatase

Rosaria Medda et al. Biochem Res Int. 2011.

Abstract

This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase. These proteins, very different in molecular weight, in primary structure, and in the catalyzed reaction, are purified using identical preliminary steps of purification and by chromatographic methods. In particular, the DEAE-cellulose chromatography is used as a useful purification step for all the four enzymes. The purification methods here reported allow to obtain a high purification of all the four proteins with a good yield. This paper will give some thorough suggestions for researchers busy in separation of macromolecules from different sources.

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Figures

Figure 1
Figure 1
Euphorbia characias, the Mediterranean spurge.
Figure 2
Figure 2
SDS-PAGE. Protein samples after the purification steps. Line 1: standard molecular weights: bovine serum albumin (66 kDa), ovalbumin (45 kDa), glyceraldehydes-3-phosphate dehydrogenase (36 kDa), carbonic anhydrase (29 kDa), trypsinogen (24 kDa), and α-lactalbumin (14.2 kDa); line 2: ELP; line 3: ELAO; line 4: ELNPP; line 5: ELPAP.
Figure 3
Figure 3
Visible absorption spectrum of homogeneous (a) Euphorbia amine oxidase (56 μM), (b) Euphorbia peroxidase (3.6 μM), and (c) Euphorbia purple acid phosphatase (62 μM). Buffer used: ELAO and ELP 100 mM K+-phosphate, pH 7.0; ELPAP 100 mM Tris/HCl, pH 7.0.
Scheme 1
Scheme 1
Steps  of the purification methods for the enzymes from Euphorbia latex. (a) ELAO; (b) ELP; (c) ELNPP; (d) ELPAP.
See this image and copyright information in PMC

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