Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2012 Mar;61(Pt 3):431-437.
doi: 10.1099/jmm.0.034744-0. Epub 2011 Oct 20.

Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010

Huu Dat Tran  1   2 , Munirul Alam  3 , Nguyen Vu Trung  1   2 , Nguyen Van Kinh  4   2 , Hong Ha Nguyen  2 , Van Ca Pham  2 , Mohammad Ansaruzzaman  3 , Shah Manzur Rashed  3 , Nurul A Bhuiyan  3 , Tuyet Trinh Dao  2 , Hubert P Endtz  5   3 , Heiman F L Wertheim  6   7
Affiliations
Comparative Study

Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010

Huu Dat Tran et al. J Med Microbiol. 2012 Mar.

Abstract

Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V. cholerae O1 strains isolated from diarrhoeal patients in northern Vietnam between 2007 and 2010 were investigated for antibiotic susceptibility and characterized by using phenotypic and genotypic tests, including PFGE analysis. Ten clinical V. cholerae O1 isolates from Bangladesh and Zimbabwe were included for comparison. The results revealed that all isolates were resistant to co-trimoxazole and nalidixic acid, 29 % were resistant to tetracycline and 1 % were resistant to azithromycin. All strains were susceptible to ampicillin-sulbactam, doxycycline, chloramphenicol and ciprofloxacin and 95 % were susceptible to azithromycin. MIC values did show reduced susceptibility to fluoroquinolones and 63 % of the strains were intermediately resistant to tetracycline. The isolates expressed phenotypic traits of both serogroup O1 Ogawa and El Tor and harboured an rstR El Tor and ctxB classical biotype. Among the outbreak isolates, only a single PFGE pattern was observed throughout the study period. This study shows that multi-drug resistant V. cholerae altered El Tor producing classical CT strains are now predominant in northern Vietnam.

PubMed Disclaimer

Figures

Fig. 1.
Fig. 1.
NotI-restricted PFGE profiles of V. cholerae O1 strains isolated from Vietnam, Zimbabwe and Bangladesh. Vietnamese strains are indicated with a V-number, PCS-12 and PCS34 are Bangladesh strains and Z-47(2009) and Z-62(2009) are Zimbabwe strains. SB, Salmonella Braenderup molecular size marker.
Fig. 2.
Fig. 2.
NotI-restricted PFGE profiles and a dendrogram reconstructed using UPGMA based on the banding patterns of V. cholerae O1 strains from Vietnam, Zimbabwe and Bangladesh. Cluster a includes all the Vietnamese strains showing 100 % similarity. Cluster b includes strains from Zimbabwe (Z-47 and Z-62) and Bangladesh (PCS-12 and PCS-34).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Alam M., Nusrin S., Islam A., Bhuiyan N. A., Rahim N., Delgado G., Morales R., Mendez J. L., Navarro A. & other authors (2010). Cholera between 1991 and 1997 in Mexico was associated with infection by classical, El Tor, and El Tor variants of Vibrio cholerae. J Clin Microbiol 48, 3666–3674 10.1128/JCM.00866-10 - DOI - PMC - PubMed
    1. Ang G. Y., Yu C. Y., Balqis K., Elina H. T., Azura H., Hani M. H., Yean C. Y. (2010). Molecular evidence of cholera outbreak caused by a toxigenic Vibrio cholerae O1 El Tor variant strain in Kelantan, Malaysia. J Clin Microbiol 48, 3963–3969 10.1128/JCM.01086-10 - DOI - PMC - PubMed
    1. Ansaruzzaman M., Bhuiyan N. A., Nair B. G., Sack D. A., Lucas M., Deen J. L., Ampuero J., Chaignat C. L., Mozambique Cholera Vaccine Demonstration Project Coordination Group (2004). Cholera in Mozambique, variant of Vibrio cholerae. Emerg Infect Dis 10, 2057–2059 - PMC - PubMed
    1. Ansaruzzaman M., Bhuiyan N. A., Safa A., Sultana M., McUamule A., Mondlane C., Wang X. Y., Deen J. L., von Seidlein L., Clemens J. (2007). Genetic diversity of El Tor strains of Vibrio cholerae O1 with hybrid traits isolated from Bangladesh and Mozambique. Int J Med Microbiol 297, 443–449 10.1016/j.ijmm.2007.01.009 - DOI - PubMed
    1. Bhattacharya T., Chatterjee S., Maiti D., Bhadra R. K., Takeda Y., Nair G. B., Nandy R. K. (2006). Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1, non-O139 strains. Environ Microbiol 8, 526–634 10.1111/j.1462-2920.2005.00932.x - DOI - PubMed

Publication types

MeSH terms

Substances