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Comparative Study
. 2011;13(5):R172.
doi: 10.1186/ar3495. Epub 2011 Oct 21.

Frequency of disease-associated and other nuclear autoantibodies in patients of the German Network for Systemic Scleroderma: correlation with characteristic clinical features

Rudolf Mierau  1 , Pia Moinzadeh, Gabriela Riemekasten, Inga Melchers, Michael Meurer, Frank Reichenberger, Michael Buslau, Margitta Worm, Norbert Blank, Rüdiger Hein, Ulf Müller-Ladner, Annegret Kuhn, Cord Sunderkötter, Aaron Juche, Christiane Pfeiffer, Christoph Fiehn, Michael Sticherling, Percy Lehmann, Rudolf Stadler, Eckhard Schulze-Lohoff, Cornelia Seitz, Ivan Foeldvari, Thomas Krieg, Ekkehard Genth, Nicolas Hunzelmann
Affiliations
Comparative Study

Frequency of disease-associated and other nuclear autoantibodies in patients of the German Network for Systemic Scleroderma: correlation with characteristic clinical features

Rudolf Mierau et al. Arthritis Res Ther. 2011.

Abstract

Introduction: In the present study, we analysed in detail nuclear autoantibodies and their associations in systemic sclerosis (SSc) patients included in the German Network for Systemic Scleroderma Registry.

Methods: Sera of 863 patients were analysed according to a standardised protocol including immunofluorescence, immunoprecipitation, line immunoassay and immunodiffusion.

Results: Antinuclear antibodies (ANA) were detected in 94.2% of patients. In 81.6%, at least one of the autoantibodies highly associated with SSc or with overlap syndromes with scleroderma features was detected, that is, anti-centromere (35.9%) or anti-topoisomerase I (30.1%), followed in markedly lower frequency by antibodies to PM-Scl (4.9%), U1-ribonucleoprotein (U1-RNP) (4.8%), RNA polymerases (RNAPs) (3.8%), fibrillarin (1.4%), Ku (1.2%), aminoacyl-transfer RNA synthetases (0.5%), To (0.2%) and U11-RNP (0.1%). We found that the simultaneous presence of SSc-associated autoantibodies was rare (1.6%). Furthermore, additional autoantibodies were detected in 55.4% of the patients with SSc, of which anti-Ro/anti-La, anti-mitochondrial and anti-p25/p23 antibodies were most frequent. The coexistence of SSc-associated and other autoantibodies was common (43% of patients). SSc-associated autoantibodies disclosed characteristic associations with clinical features of patients, some of which were previously not acknowledged.

Conclusions: This study shows that five autoantigens (that is, centromere, topoisomerase I, PM-Scl, U1-RNP and RNAP) detected more than 95% of the known SSc-associated antibody responses in ANA-positive SSc patients and characterise around 79% of all SSc patients in a central European cohort. These data confirm and extend previous data underlining the central role of the determination of ANAs in defining the diagnosis, subset allocation and prognosis of SSc patients.

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Figures

Figure 1
Figure 1
Protocol for serological analysis of systemic sclerosis patient sera.
See this image and copyright information in PMC

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