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. 2011 Oct 23;8(12):1037-40.
doi: 10.1038/nmeth.1740.

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes

David A Elliott  1 , Stefan R Braam, Katerina Koutsis, Elizabeth S Ng, Robert Jenny, Ebba L Lagerqvist, Christine Biben, Tanya Hatzistavrou, Claire E Hirst, Qing C Yu, Rhys J P Skelton, Dorien Ward-van Oostwaard, Sue Mei Lim, Ouda Khammy, Xueling Li, Susan M Hawes, Richard P Davis, Adam L Goulburn, Robert Passier, Owen W J Prall, John M Haynes, Colin W Pouton, David M Kaye, Christine L Mummery, Andrew G Elefanty, Edouard G Stanley
Affiliations

NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes

David A Elliott et al. Nat Methods. .

Abstract

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.

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References

    1. Stem Cells. 2007 Sep;25(9):2206-14 - PubMed
    1. Nature. 2008 May 22;453(7194):524-8 - PubMed
    1. Differentiation. 2008 Apr;76(4):357-70 - PubMed
    1. Nat Protoc. 2008;3(5):768-76 - PubMed
    1. Cell. 2007 Mar 9;128(5):947-59 - PubMed

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