doi: 10.1016/0022-1759(90)90192-x.
Evaluation of dissociation constants of antigen-antibody complexes by ELISA
Affiliations
- PMID: 2202761
- DOI: 10.1016/0022-1759(90)90192-x
Item in Clipboard
Evaluation of dissociation constants of antigen-antibody complexes by ELISA
J Immunol Methods.
.
Abstract
In this communication some of the advantages and constraints in the use of ELISA (enzyme-linked immunosorbent assay) procedures to evaluate antigen-antibody dissociation constants (Kd) are discussed and experimental conditions under which the effective Kd is close to the true value are proposed. Interactions between horseradish peroxidase (POD), human myoglobin and insulin with mono- and polyclonal antibodies (McAb and PcAb) were used to demonstrate that ELISA can be used to determine the average Kd, characterizing the interaction between antigens and PcAb. The Kd values obtained by ELISA were similar to those determined by luminescent immuno-cofactor analysis (LICA).
Similar articles
-
Measurement of the dissociation rate constant of antigen/antibody complexes in solution by enzyme-linked immunosorbent assay.J Immunol Methods. 1994 Apr 15;170(2):167-75. doi: 10.1016/0022-1759(94)90392-1. J Immunol Methods. 1994. PMID: 8157995
-
Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay.J Immunol Methods. 1985 Mar 18;77(2):305-19. doi: 10.1016/0022-1759(85)90044-4. J Immunol Methods. 1985. PMID: 3981007
-
Enzyme-linked immunosorbent assay for human insulin-like growth factor-I using monoclonal and polyclonal antibodies with defined epitope recognition.J Endocrinol. 1990 May;125(2):327-35. doi: 10.1677/joe.0.1250327. J Endocrinol. 1990. PMID: 1695662
-
Determination of dissociation constants of anti-ALP monoclonal antibodies by an ELISA - based method.Hum Antibodies. 2014;23(1-2):1-5. doi: 10.3233/HAB-140276. Hum Antibodies. 2014. PMID: 25812696
-
Single-step sandwich immunoassay of myoglobin with bifunctional monoclonal antibodies.Biochemistry (Mosc). 1999 Jun;64(6):639-47. Biochemistry (Mosc). 1999. PMID: 10395978
Cited by
-
A 3D Surface Plot for the Effective Visualization of Specific Serum Antibody Binding Properties.Antibodies (Basel). 2025 Aug 13;14(3):68. doi: 10.3390/antib14030068. Antibodies (Basel). 2025. PMID: 40843680 Free PMC article.
-
Analytical Method for Experimental Validation of Computer-Designed Antibody.Methods Mol Biol. 2023;2552:409-433. doi: 10.1007/978-1-0716-2609-2_23. Methods Mol Biol. 2023. PMID: 36346606
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
