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. 2011 Oct;4(5):346-50.
doi: 10.1111/j.1752-8062.2011.00318.x.

Expression of Neuropeptide Y, Substance P, and their receptors in the right atrium of diabetic patients

Asma Ejaz  1 , Frank W LoGerfo, Kamal Khabbaz, Leena Pradhan
Affiliations

Expression of Neuropeptide Y, Substance P, and their receptors in the right atrium of diabetic patients

Asma Ejaz et al. Clin Transl Sci. 2011 Oct.

Abstract

Objective: To investigate the expression of neuropeptides and their receptors that play a role in cardiac homeostasis in the right atrium of nondiabetic and diabetic patients undergoing coronary artery bypass graft surgery.

Background: The cardioactive neuropeptides and their receptors investigated in this study were Neuropeptide Y (NPY), and its receptors, NPY Receptor1 (NPY1R), NPY Receptor2 (NPY2R), NPY Receptor5 (NPY5R) and Substance P (SP) and its receptor, Neurokinin1R (NK1R).

Methods: The gene and protein expression of NPY, NPY1R, NPY2R, NPY5R, SP and NK1R from the atrial tissue of 10 nondiabetic and diabetic patients undergoing coronary artery bypass grafting (CABG) was assessed by Q-RTPCR, immunohistochemistry, Western blot, and ELISA.

Results: Gene expression of NPY2R, NPY5R, preproTachykinin A (SP gene), and NK1R and their respective protein expression were significantly reduced whereas that of NPY and NPY1R were unchanged in the right atrium of diabetic patients compared to nondiabetic patients.

Conclusions: These results demonstrate that the expression of neuropeptides and their receptors in the diabetic heart is significantly impaired, and may be the link between neuropathy and cardiac complications. Further studies are warranted to delineate pathophysiologic mechanisms associated with dysregulation of the cardiac neuropeptide system and the relationship to cardiac complications in diabetes.

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Figures

Figure 1
Figure 1
Gene expression of neuropeptides Y (preproNPY) and its receptors, NPY1R, NPY2R, and NPY5R and Substance P (preproTachykinin A), and its receptor NK1R in human right atrial muscle. Fold change in gene expression in diabetic patients compared to nondiabetic patients. Data are expressed as mean ± SD (N = 10).
Figure 2
Figure 2
Protein expression of neuropeptides Y (NPY) and its receptor receptors, NPY1R, NPY2R, and NPY5R in human right atrial muscle. (A) Representative IHC images of NPY, NPY1R, NPY2R, and NPY5R of nondiabetic and diabetic atrial tissue (magnification 20 ×). (B) Quantification of NPY, NPY1R, NPY2R, and NPY5R IHC protein expression using Volocity software. Data are expressed as mean ± SD (N = 10).
Figure 2
Figure 2
Protein expression of neuropeptides Y (NPY) and its receptor receptors, NPY1R, NPY2R, and NPY5R in human right atrial muscle. (A) Representative IHC images of NPY, NPY1R, NPY2R, and NPY5R of nondiabetic and diabetic atrial tissue (magnification 20 ×). (B) Quantification of NPY, NPY1R, NPY2R, and NPY5R IHC protein expression using Volocity software. Data are expressed as mean ± SD (N = 10).
Figure 3
Figure 3
Protein expression of neuropeptides Y (NPY) and its receptor receptors, NPY1R, NPY2R, and NPY5R in human right atrial muscle. (A) Representative IHC images of NPY, NPY1R, NPY2R, and NPY5R of nondiabetic and diabetic atrial tissue (magnification 20 ×). (B) Quantification of NPY, NPY1R, NPY2R, and NPY5R IHC protein expression using Volocity software. Data are expressed as mean ± SD (N = 10).
Figure 3
Figure 3
Protein expression of neuropeptides Y (NPY) and its receptor receptors, NPY1R, NPY2R, and NPY5R in human right atrial muscle. (A) Representative IHC images of NPY, NPY1R, NPY2R, and NPY5R of nondiabetic and diabetic atrial tissue (magnification 20 ×). (B) Quantification of NPY, NPY1R, NPY2R, and NPY5R IHC protein expression using Volocity software. Data are expressed as mean ± SD (N = 10).
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References

    1. Fonarow GC. An approach to heart failure and diabetes mellitus. Am J Cardiol. 2005; 96(4A): 47E–52E. - PubMed
    1. Ninds FS. Peripheral Neuropathy. Publication No. 04–4853 , 2009, http://www.ninds.nih.gov/disorders/peripheralneuropathy/detail_periphera.... Accessed June 27, 2011.
    1. Blomqvist AG, Herzog H. Y‐receptor subtypes–how many more? Trends Neurosci. 1997; 20(7): 294–298. - PubMed
    1. Richardson RJ, Grkovic I, Anderson CR. Immunohistochemical analysis of intracardiac ganglia of the rat heart. Cell Tissue Res. 2003; 314(3): 337–350. - PubMed
    1. Chottova Dvorakova M, Wiegand S, Pesta M, Slavikova J, Grau V, Reischig J, Kuncova J, Kummer W. Expression of neuropeptide Y and its receptors Y1 and Y2 in the rat heart and its supplying autonomic and spinal sensory ganglia in experimentally induced diabetes. Neuroscience. 2008; 151(4): 1016–1028. - PubMed

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