Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;34(6):1075-8.
doi: 10.1128/AAC.34.6.1075.

Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs

S Merali  1 , Y Zhang, D Sloan, S Meshnick
Affiliations

Inhibition of Pneumocystis carinii dihydropteroate synthetase by sulfa drugs

S Merali et al. Antimicrob Agents Chemother. 1990 Jun.

Abstract

A new reversed-phase high-pressure liquid chromatography assay procedure for dihydropteroate synthetase (DHPS) that involves the elution of the enzyme incubation solution with a series of three solvents of decreasing polarity (ammonium phosphate buffer, 10% methanol, and 50% methanol) was designed. By this procedure DHPS was detected in Escherichia coli and Pneumocystis carinii with specific activities of 450 and 14 U/mg, respectively. A comparison of the effects of five sulfa drugs on P. carinii DHPS activity revealed that dapsone is the most potent of these drugs.

PubMed Disclaimer

References

    1. Anal Biochem. 1970 Jan;33(1):114-9 - PubMed
    1. Antimicrob Agents Chemother. 1973 Jun;3(6):665-9 - PubMed
    1. Antimicrob Agents Chemother. 1974 Mar;5(3):289-93 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Methods Enzymol. 1980;66:560-4 - PubMed

Publication types

LinkOut - more resources