Simultaneous determination of eleven major flavonoids in the pollen of Typha angustifolia by HPLC-PDA-MS

Abstract

Introduction: The pollen of Typha angustifolia L. has been used for the treatment of dysmenorrhea, stranguria and metrorrhagia. Flavonoids are major active compounds in this pollen and their quantification is important for its quality control.

Objective: To establish an HPLC-PDA-MS method for simultaneous determination of the 11 majority flavonoids in the pollen of T. angustifolia.

Methodology: The optimal condition of separation was achieved on a reversed-phase C₁₈ column with gradient elution of acetonitrile and 0.05% formic acid (v/v) at a flow-rate of 0.8 mL/min; the column temperature was set at 35 °C.

Results: All calibration curves showed good linear regression (r² > 0.9992). The method provided good accuracy, precision, recovery and sensitivity for the quantification of the 11 compounds analysed.

Conclusion: The HPLC method established is appropriate for the quality assurance of the pollen of T. angustifolia.