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. 1990 Aug 25;18(16):4659-64.
doi: 10.1093/nar/18.16.4659.

Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes

L Szilák  1 , P Venetianer, A Kiss
Affiliations
Free PMC article

Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes

L Szilák et al. Nucleic Acids Res. .
Free PMC article

Abstract

The genes coding for the GGNCC specific Sau96I restriction and modification enzymes were cloned and expressed in E. coli. The DNA sequence predicts a 430 amino acid protein (Mr: 49,252) for the methyltransferase and a 261 amino acid protein (Mr: 30,486) for the endonuclease. No protein sequence similarity was detected between the Sau96I methyltransferase and endonuclease. The methyltransferase contains the sequence elements characteristic for m5C-methyltransferases. In addition to this, M.Sau96I shows similarity, also in the variable region, with one m5C-methyltransferase (M.SinI) which has closely related recognition specificity (GGA/TCC). M.Sau96I methylates the internal cytosine within the GGNCC recognition sequence. The Sau96I endonuclease appears to act as a monomer.

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References

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