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. 2010 Jul;25(3):168-72.
doi: 10.5001/omj.2010.51.

Association between seminal plasma copper and magnesium levels with oxidative stress in iraqi infertile men

Omar F Abdul-Rasheed  1
Affiliations

Association between seminal plasma copper and magnesium levels with oxidative stress in iraqi infertile men

Omar F Abdul-Rasheed. Oman Med J. 2010 Jul.

Abstract

Objectives: To study the association between copper, magnesium and malondialdehyde levels in seminal plasma of oligozoospermic, azoospermic in relation to normozoospermic men.

Methods: The present study was conducted at the Chemistry and Biochemistry department, College of Medicine, Al-Nahrain University, Baghdad-Iraq during September 2007 to February 2008 after obtaining approval from the research and ethics committee and obtaining written consent, 78 infertile men (age range 33.01±4.20 years) were recruited at the institute of embryo research and infertility treatment, Al-Kadhimiya teaching hospital, Iraq and were categorized according to their seminal fluid parameters to oligozoospermia (n=43) and azoospermia (n=35). 41 fertile men (age range 30.29±2.30 years) were selected as controls. Seminal plasma copper and magnesium were measured by atomic absorption spectrophotometry. Malondialdehyde was measured calorimetrically using thiobarbituric acid assay which detects thiobarbituric acid reactive substances.

Results: Seminal plasma copper level was decreased significantly (p=0.000) in the azoospermic group compared to the control group. Whereas, the level decreased non-significantly in the oligozoospermic group. Seminal plasma magnesium levels were decreased significantly (p=0.000) in all the infertility groups studied. On the other hand, malondialdehyde levels which is an end product of lipid peroxidation were significantly elevated (p=0.000) in all the infertility groups studied.

Conclusion: Copper and magnesium work in different ways in order to maintain normal environment for spermatozoa for normal fertilization to occur.

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Figures

Figure 1
Figure 1
Pearson’s Correlation Plot of Seminal Plasma Copper levels versus Normal Sperm Morphology in control group
Figure 2
Figure 2
Pearson’s orrelation plot of seminal plasma copper levels versus normal sperm morphology in patients with oligozoospermia
Figure 3
Figure 3
Pearson’s correlation plot of seminal plasma magnesium levels versus normal sperm morphology in oligozoospermic infertility group
See this image and copyright information in PMC

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