Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;3(1):45-7.
doi: 10.1007/BF01141177.

An EXAFS study of the copper accumulated by yeast cells

A Desideri  1 , H J Hartmann, S Morante, U Weser
Affiliations

An EXAFS study of the copper accumulated by yeast cells

A Desideri et al. Biol Met. 1990.

Abstract

X-ray absorption spectroscopy has been applied to the in vivo examination of copper-resistant yeast cells. The in vivo structure of the metal-binding site of the accumulated copper has been compared to that of the purified yeast thionein. Analysis of the EXAFS spectra performed on intact yeast cells indicates that the accumulated copper is univalent and is exclusively coordinated to sulfur atoms at a distance of 219 pm with an average coordination number of 2. In contrast, the purified protein indicates a univalent copper trigonally coordinated to sulfur at a distance of 221 pm. These discrepancies are discussed in terms of copper location in the resistant yeast cells.

PubMed Disclaimer

References

    1. J Biol Chem. 1988 Jun 15;263(17):8199-203 - PubMed
    1. J Biol Chem. 1989 Apr 5;264(10):5598-605 - PubMed
    1. J Biol Chem. 1986 Dec 25;261(36):16895-900 - PubMed
    1. Biochem J. 1986 Jun 1;236(2):585-9 - PubMed
    1. Biochim Biophys Acta. 1988 Mar 2;953(1):1-5 - PubMed

Publication types