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. 2012 Jan;81(1):15-6.
doi: 10.1002/cyto.a.21158. Epub 2011 Nov 3.

OMIP-004: in-depth characterization of human T regulatory cells

Angélique Biancotto  1 , Pradeep K Dagur, J Chris Fuchs, Marc Langweiler, J Philip McCoy Jr
Affiliations

OMIP-004: in-depth characterization of human T regulatory cells

Angélique Biancotto et al. Cytometry A. 2012 Jan.
No abstract available

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Figures

Figure 1
Figure 1
An example of staining patterns and gating strategies for all fluorescent parameters. PBMC were stained according to the OMIP-004 protocol. (A) Debris and doublets were excluded from analysis. Live mononuclear cells were selected for sequential analysis. T cells were identified by CD3 expression. T cells subsets were identified by expression of CD4 and/or CD8. The CD4 subset was examined for expression of CD25 and Foxp3. (B) Representation of remaining parameters, in CD8+ T cells (left), Treg (center) and CD4+ T cells (right). (C) Expression of CCR7 and CD127 on naive CD8+ (left) and naive CD4+ T cells (right). Naive cells were gated as indicated in Figure 1A, through CD45RA and CD27 bivariate dot plots. Numbers in green next to the dot plots are a reference to the populations reported in online Supporting Information Table 6.
See this image and copyright information in PMC

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