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. 1990 Aug;123(2):179-85.
doi: 10.1111/j.1365-2133.1990.tb01845.x.

The extension technique: a new method of demonstrating initial lymph vessels in excised human skin

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The extension technique: a new method of demonstrating initial lymph vessels in excised human skin

D Lubach et al. Br J Dermatol. 1990 Aug.

Abstract

The initial lymph vessels, the lymph capillaries and precollectors, are difficult to detect in normal skin using routine histological methods. A method is described in which specimens of the skin are fixed in an expanded condition. In 1.05 microns thick plastic-embedded sections the dilated lymph vessels of the skin can be demonstrated. The dermal lymph vessel network can be seen following the subepidermal injection of a coloured solution. The endothelial cells and subendothelial fibrous network can be examined using electron microscopy.

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