Diarrhea induced by Treponema hyodysenteriae: a young chick cecal model for swine dysentery

Abstract

The experimental infection of 25 young chicks with Treponema hyodysenteriae was carried out. Treponemes were isolated from 21 of 25 chicks on day 21 after inoculation. The ceca of chicks infected with treponemes were atrophied. The lumen was filled with a white watery fluid instead of digested feed. In some infected chicks, a cecal core was observed with the fluid in the cecum. The cecal core was grayish, hard, and rod shaped. It consisted of eroded cells and debris of treponemes and resembled the pseudomembrane. Bloody mucus was also observed in one chick. The thickness of the mucosae in 17 of 25 chicks were markedly increased. The histological changes were classified into two types. In the case of regressive changes of epithelial cells which mean severe erosion, the laminae propriae were exposed. Hemorrhage, edema, and heterophil infiltration in the laminae propriae were also confirmed. Numerous treponemes were observed within the edematous area under the remaining epithelia and also invaded the epithelial cells and laminae propriae. In the other case, progressive changes, that is, hyperplasia of mucosal epithelial cells and elongation of the crypt, were observed. The epithelia consisted mainly of cuboidal basophilic cells, mitotic cells, and goblet cells. The mitotic cells increased in number and were also observed near the superficial luminal surface of the ceca. Mucous goblet cells were also considerably increased in number. The erosion of superficial luminal epithelial cells was not so severe, but edema in laminae propriae was frequently observed. Electron-microscopic observation demonstrated that the basophilic epithelial cells were polyribosome rich, mitochondria poor, and lipid droplet poor. Furthermore, tonofibril-like structures under the terminal web in cytoplasms were lost, and numerous membrane-bound vesicles at the terminal web with free ribosomes were observed. In places, a number of vesicles were observed between microvilli, and some vesicles were released from the apical cytoplasm of epithelial cells. Microvilli also became scarce and irregular. The junctional complexes between cuboidal cells became unclear, the interdigitations between cuboidal cells became loose, and the intercellular spaces were widened. In conclusion, the appearance of numerous membrane-bound vesicles at the terminal web and the dilated intercellular space indicates increased secretion of water and electrolytes, and the abnormal proliferation of such immature epithelial cells indicates impaired absorption. On the basis of our findings, we can say that diarrhea induced by T. hyodysenteriae can be developed by synergism between impaired absorption and increased secretion by intestinal mucosae with hyperplastic immature epithelial cells.(ABSTRACT TRUNCATED AT 400 WORDS)