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. 1990 Sep 11;18(17):5051-3.
doi: 10.1093/nar/18.17.5051.

The repair patch of E. coli (A)BC excinuclease

Sibghat-Ullah  1 , A Sancar, J E Hearst
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Free PMC article

The repair patch of E. coli (A)BC excinuclease

Sibghat-Ullah et al. Nucleic Acids Res. .
Free PMC article

Abstract

The size of repair patch made by E. coli DNA polymerase I (Poll) following the removal of a thymine-psoralen monoadduct by E. coli (A)BC excinuclease was determined by using an M13mp19 DNA with a single psoralen monoadduct at the polylinker region. Incubation of this substrate with (A)BC excinuclease, Poll and a combination of 3 dnTP plus 1 dNTP(alpha S) for each nucleotide, and DNA ligase resulted in a repair patch with phosphorothioate linkages. The preferential hydrolysis of phosphorothioate bonds by heating in iodoethanol revealed a patch size--with minimal nick translation--equal in length to the 12 nucleotide gap generated by this excision nuclease.

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