doi: 10.1007/BF00423327.
Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose-specific enzyme from Klebsiella pneumoniae
Affiliations
- PMID: 2206103
- DOI: 10.1007/BF00423327
Item in Clipboard
Construction of a new catabolic pathway for D-fructose in Escherichia coli K12 using an L-sorbose-specific enzyme from Klebsiella pneumoniae
Arch Microbiol.
1990.
Abstract
Starting with a fruK (formerly fpk) mutant of Escherichia coli K12 lacking D-fructose-1-phosphate kinase (E.C. 2.7.1.3.), fructose positive derivatives were isolated after introduction of the cloned gene sorE from Klebsiella pneumoniae coding for an L-sorbose-1-phosphate reductase. The new pathway was shwon to proceed from D-fructose via D-fructose-1-phosphate and D-mannitol-1-phosphate to D-fructose 6-phosphate. It involves a transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from K. pneumoniae respectively.
Similar articles
-
Cloning and physical mapping of the sor genes for L-sorbose transport and metabolism from Klebsiella pneumoniae.Mol Microbiol. 1990 Sep;4(9):1557-65. doi: 10.1111/j.1365-2958.1990.tb02067.x. Mol Microbiol. 1990. PMID: 2287279
-
L-Sorbose metabolism in Klebsiella pneumoniae and Sor+ derivatives of Escherichia coli K-12 and chemotaxis toward sorbose.J Bacteriol. 1984 Jan;157(1):39-45. doi: 10.1128/jb.157.1.39-45.1984. J Bacteriol. 1984. PMID: 6361004 Free PMC article.
-
The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026.FEMS Microbiol Lett. 2001 Jan 15;194(2):221-7. doi: 10.1111/j.1574-6968.2001.tb09473.x. FEMS Microbiol Lett. 2001. PMID: 11164312
-
Routes for fructose utilization by Escherichia coli.J Mol Microbiol Biotechnol. 2001 Jul;3(3):355-9. J Mol Microbiol Biotechnol. 2001. PMID: 11361065 Review.
-
If at first you don't succeed em leaderfructose utilization by Escherichia coli.Adv Enzyme Regul. 2002;42:349-60. doi: 10.1016/s0065-2571(01)00038-3. Adv Enzyme Regul. 2002. PMID: 12123725 Review. No abstract available.
Cited by
-
Molecular analysis of the phosphoenolpyruvate-dependent L-sorbose: phosphotransferase system from Klebsiella pneumoniae and of its multidomain structure.Mol Gen Genet. 1995 Mar 10;246(5):610-8. doi: 10.1007/BF00298968. Mol Gen Genet. 1995. PMID: 7700234
-
Cloning of the Escherichia coli sor genes for L-sorbose transport and metabolism and physical mapping of the genes near metH and iclR.J Bacteriol. 1992 Dec;174(23):7784-90. doi: 10.1128/jb.174.23.7784-7790.1992. J Bacteriol. 1992. PMID: 1447144 Free PMC article.
-
Positive and negative regulation of expression of the L-sorbose (sor) operon by SorC in Klebsiella pneumoniae.Mol Gen Genet. 1990 Nov;224(2):193-200. doi: 10.1007/BF00271552. Mol Gen Genet. 1990. PMID: 2277638