Capillary gel electrophoresis versus SDS PAGE of exudate from fresh pork

Abstract

This investigation compared electrophoretic profiles of exudate from fresh normal pork in which the protein separation was undertaken by both sodium dodecyl sulphate polyacrylamide gel electrophoresis SDS PAGE and capillary gel electrophoresis. The profiles obtained using the two techniques were similar and in many investigations either method would be suitable. It is suggested that capillary gel electrophoresis offers a number of benefits over the traditional time consuming and labour intensive gel electrophoresis techniques which involves gel preparation, sample application, staining and eventually quantification of the zones by densitometry. For separation of proteins in meat-based systems the advantages of capillary gel electrophoresis include on-capillary detection, instrumental automation, rapid analysis time and low sample volumes while providing similar information to that obtained from SDS PAGE.