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. 2011 Nov 13:8:517.
doi: 10.1186/1743-422X-8-517.

Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China

Shao-Lun Zhai  1 , Sheng-Nan Chen, Zu-Zhang Wei, Jian-Wu Zhang, Lv Huang, Tao Lin, Cheng Yue, Duo-Liang Ran, Shi-Shan Yuan, Wen-Kang Wei, Jin-Xue Long
Affiliations

Co-existence of multiple strains of porcine circovirus type 2 in the same pig from China

Shao-Lun Zhai et al. Virol J. .

Abstract

Pigs are often co-infected by different viral strains from the same virus. Up to now, there are few reports about co-existence of different porcine circovirus type 2 (PCV2) strains in China. The aim of this study was to evaluate it in Chinese swine herds. 118 PCV2 positive DNAs isolated from diseased pigs identified by classic PCR were re-detected using a modified differential PCR assay. The results indicated that co-existence rates of PCV2 were 32.2% (38/118) in diseased pigs and 0% (0/41) in asymptomatic pigs. Four PCV2 complete genomes were cloned from two co-infected samples and their nucleotide (nt) identities were 95%-97.3%. The phylogenetic analysis showed that four PCV2 strains were divided into different genotypes, PCV2a, PCV2b, PCV2d and PCV2e, respectively. In addition, co-existence were not detected in 41 serum samples from healthy pigs but PCV2 single infection (31.7%, 13/41) existed. These data revealed that the co-existence of different strains of PCV2 might contribute to the development of more severe clinical symptoms for pigs. This is the first report confirming the co-existence of different PCV2 strains in Chinese swine herds. Meanwhile, this study could help us to understand new infection and prevalence forms of PCV2 clinically.

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Figures

Figure 1
Figure 1
Genome organization (A) and phylogenetic analysis (B) of PCV2 strains generated in this study. Note: PCV2a reference strain 1010-Stoon (Accession No. AF055392) labeled with '•', PCV2b reference strain 48285 (Accession No. AF055394) labeled with '■', PCV2c reference strain DK1987PMWSfree (Accession No. EU148504) labeled with '▲', PCV2d reference strain TJ (Accession No. AY181946) labeled with '□', PCV2e reference strain GX0601 (Accession No. EF524532) labeled with 'Δ'.
Figure 2
Figure 2
Sequence alignment results about coding aa sequences of capsid gene (ORF2).
See this image and copyright information in PMC

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