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. 2012 Jan;56(1):559-62.
doi: 10.1128/AAC.05289-11. Epub 2011 Nov 14.

Genetic features of the widespread plasmid coding for the carbapenemase OXA-48

Laurent Poirel  1 , Rémy A Bonnin, Patrice Nordmann
Affiliations

Genetic features of the widespread plasmid coding for the carbapenemase OXA-48

Laurent Poirel et al. Antimicrob Agents Chemother. 2012 Jan.

Abstract

Complete sequencing of plasmid pOXA-48a carrying the bla(OXA-48) gene from a Klebsiella pneumoniae isolate was performed. Its backbone corresponded to that of an IncL/M-type plasmid, in which the bla(OXA-48) gene had been integrated through the acquisition of the Tn1999 composite transposon without any other antibiotic resistance gene. Molecular epidemiology using a collection of international OXA-48 producers revealed the wide diffusion of pOXA-48a or closely related plasmids.

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Figures

Fig 1
Fig 1
Major structural features of plasmid pOXA-48a from K. pneumoniae 11978 (22) in comparison with the IncL/M plasmids pCTX-M-3 (11) and pNDM-HK (14). White boxes indicate plasmid scaffold regions that are in common among plasmids. The locus Tra within the box is indicated. Resistance genes are indicated by orange boxes, except the β-lactamase genes, which are indicated by blue boxes. Transposon-related genes (tnpA, tnpR, and tnpM) and insertion sequences are indicated by red boxes. Replicase genes are indicated by purple boxes.
Fig 2
Fig 2
Genetic environment and content of Tn1999. The different gene names are indicated. The sequence of the 9-bp target site duplication is underlined.
See this image and copyright information in PMC

References

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