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. 2012 Feb;56(2):666-71.
doi: 10.1128/AAC.05305-11. Epub 2011 Nov 14.

The class A β-lactamase FTU-1 is native to Francisella tularensis

Nuno T Antunes  1 , Hilary Frase, Marta Toth, Sergei B Vakulenko
Affiliations

The class A β-lactamase FTU-1 is native to Francisella tularensis

Nuno T Antunes et al. Antimicrob Agents Chemother. 2012 Feb.

Abstract

The class A β-lactamase FTU-1 produces resistance to penicillins and ceftazidime but not to any other β-lactam antibiotics tested. FTU-1 hydrolyzes penicillin antibiotics with catalytic efficiencies of 10(5) to 10(6) M(-1) s(-1) and cephalosporins and carbapenems with catalytic efficiencies of 10(2) to 10(3) M(-1) s(-1), but the monobactam aztreonam and the cephamycin cefoxitin are not substrates for the enzyme. FTU-1 shares 21 to 34% amino acid sequence identity with other class A β-lactamases and harbors two cysteine residues conserved in all class A carbapenemases. FTU-1 is the first weak class A carbapenemase that is native to Francisella tularensis.

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Figures

Fig 1
Fig 1
Comparison of the amino acid sequences of putative class A carbapenemases from Francisella tularensis subsp. holarctica LVS (FTU-1 and CAJ79396) and those of known class A carbapenemases (NMC-A, KPC-2, SME-1, SFC-1, and BIC-1) and weak carbapenemases (BEL-1 and GES-1). The conserved 70SXXK73, 130SDN132, 166EXXXN170, and 234KTG236 motifs are shown in bold. Highlighted in gray are the conserved cysteine residues, Cys69 and Cys238. The leader sequence of FTU-1, which is removed to give the mature enzyme, is underlined.
Fig 2
Fig 2
Alignment of FTU-1 and its homologs in F. tularensis genomes. The arrows indicate the conserved Cys69 and Cys238. The positions where the amino acid sequence varies are underlined. In the following descriptions of the proteins, accession numbers are given in parentheses. (a) FTU-1 is found in F. tularensis subsp. holarctica strains LVS (CAJ79318), FSC200 (ZP_02275799.1), FTNF002-00 (YP_001428362.1), 257 (ZP_04983583.1), OSU18 (YP_763419.1), FSC022 (ZP_04985240.1), and URFT1 (ZP_06558201.1). (b) FTU-1 homolog from F. tularensis subsp. mediasiatica FSC147 (YP_001891444.1). (c) FTU-1 homolog from F. tularensis subsp. tularensis WY96-3418 (YP_001122050.1). (d) FTU-1 homolog from F. tularensis subsp. tularensis strains FSC198 (YP_666764.1), FSC033 (ZP_04986245), SCHU S4 (YP_169632.1), and MA00-2987 (ZP_05247260.1). (e) FTU-1 homolog from F. tularensis subsp. novicida U112 (YP_898713.1).
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References

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