Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Oct;36(10):1815-9.

Improved method for identifying alpha 1-antitrypsin Pi M subtypes by isoelectric focusing in agarose

M L Nowicki  1 , E F Freier
Affiliations
  • PMID: 2208661
Comparative Study

Improved method for identifying alpha 1-antitrypsin Pi M subtypes by isoelectric focusing in agarose

M L Nowicki et al. Clin Chem. 1990 Oct.

Abstract

We describe an improved method for the classification of alpha 1-antitrypsin variants by isoelectric focusing in agarose. Identification of the three Pi M subtypes can now be made by using a narrow-range carrier ampholyte (pH 4.2-4.9) and pretreating serum with dithioerythritol-iodoacetic acid to enhance band resolution. Phenotype results for two groups of Pi M homo- and heterozygotes are compared to illustrate the improved accuracy of the new method.

PubMed Disclaimer

Publication types

LinkOut - more resources