Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption

Abstract

The Chaihu-Shaoyao drug pair (Bupleuri Radix and Paeoniae Radix Alba) which is a traditional Chinese drug pair, has been widely used for anti-inflammatory purposes. Saikosaponin a (SSA), saikosaponin d (SSD) and paeoniflorin are identified as the main components in the pair. The present study focused on the interaction of the main components based on investigating their intestinal absorption using a four-site perfused rat intestinal model in order to clarify the mechanism of the compatibility of Chaihu-Shaoyao. The concentrations of SSA, SSD and paeoniflorin in the intestinal perfusate were determined by LC/MS or UPLC (Ultra Performance Liquid Chromatography) methods, followed by P*(eff) (effective permeability) and 10% ABS (the percent absorption of 10 cm of intestine) calculations. The results showed that all of the three main components displayed very low permeabilities (P*(eff) < 0.4), which implied their poor absorption in the rat intestine. The absorption levels of SSA and SSD were similar in intestine and higher in ileum than those in other intestinal regions in the decreasing order: colon, jejunum and duodenum. However, there is no significant difference in the absorption of paeoniflorin in the four segments (P < 0.05). The P*(eff) values of paeoniflorin exhibited an almost 2.11-fold or 1.90-fold increase in ileum when it was co-administrated with SSA and SSD, as well as 2.42-, 2.18-fold increase in colon, respectively, whereas the absorptions of SSA and SSD were not influenced by paeoniflorin. In conclusion, SSA and SSD could promote the absorption of paeoniflorin. To some extent this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.

Figure 1

Chemical structures of SSA (A) SSD (B) and paeoniflorin (C).

Figure 2

Effective permeability of paeoniflorin in different intestinal segments. (a) paeoniflorin alone; (b) paeoniflorin co-administration with SSA; (c) paeoniflorin co-administration with SSD. P < 0.05 vs. paeoniflorin alone.

Figure 3

Effective permeability of SSA and SSD in different intestinal segments ( ± s, n = 4). Figure (A) SSA; (a) alone; (b) co-administration with paeoniflorin; Figure (B) SSD; (c) alone; (d) co-administration with paeoniflorin.