Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1990 Oct;9(10):3233-40.
doi: 10.1002/j.1460-2075.1990.tb07522.x.

p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes

J Shuttleworth  1 , R Godfrey, A Colman
Affiliations
Comparative Study

p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes

J Shuttleworth et al. EMBO J. 1990 Oct.

Abstract

The clone MO15 which codes for a 40 kd protein (p40MO15) with 40% amino acid identity to the human cdc2 protein kinase has been isolated from a Xenopus cDNA library using a synthetic oligonucleotide probe. MO15 mRNA is accumulated during oogenesis, becomes de-adenylated during meiotic maturation, and is degraded after the mid-blastula-transition stage of embryogenesis. Translation of p40MO15 is restricted to non-mature oocytes. Specific inhibition of p40MO15 synthesis in stage VI oocytes by antisense oligonucleotide depletion of MO15 mRNA increases the rate of progesterone induced H1 kinase activation and oocyte maturation. This effect can be reversed by subsequent injection of synthetic MO15 mRNA. These results suggest that p40MO15 is involved in negatively regulating meiosis.

PubMed Disclaimer

References

    1. Exp Cell Res. 1975 Mar 15;91(2):381-8 - PubMed
    1. Curr Opin Cell Biol. 1989 Apr;1(2):268-74 - PubMed
    1. J Biol Chem. 1977 Mar 10;252(5):1712-8 - PubMed
    1. Proc R Soc Lond B Biol Sci. 1977 Sep 5;198(1132):211-47 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1176-80 - PubMed

Publication types

MeSH terms

Associated data