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. 2011 Nov;17(11):2122-9.
doi: 10.3201/eid1711.110805.

Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011

Deborah Talkington  1 , Cheryl Bopp, Cheryl Tarr, Michele B Parsons, Georges Dahourou, Molly Freeman, Kevin Joyce, Maryann Turnsek, Nancy Garrett, Michael Humphrys, Gerardo Gomez, Steven Stroika, Jacques Boncy, Benjamin Ochieng, Joseph Oundo, John Klena, Anthony Smith, Karen Keddy, Peter Gerner-Smidt
Affiliations

Characterization of toxigenic Vibrio cholerae from Haiti, 2010-2011

Deborah Talkington et al. Emerg Infect Dis. 2011 Nov.

Abstract

In October 2010, the US Centers for Disease Control and Prevention received reports of cases of severe watery diarrhea in Haiti. The cause was confirmed to be toxigenic Vibrio cholerae, serogroup O1, serotype Ogawa, biotype El Tor. We characterized 122 isolates from Haiti and compared them with isolates from other countries. Antimicrobial drug susceptibility was tested by disk diffusion and broth microdilution. Analyses included identification of rstR and VC2346 genes, sequencing of ctxAB and tcpA genes, and pulsed-field gel electrophoresis with SfiI and NotI enzymes. All isolates were susceptible to doxycycline and azithromycin. One pulsed-field gel electrophoresis pattern predominated, and ctxB sequence of all isolates matched the B-7 allele. We identified the tcpETCIRS allele, which is also present in Bangladesh strain CIRS 101. These data show that the isolates from Haiti are clonally and genetically similar to isolates originating in Africa and southern Asia and that ctxB-7 and tcpET(CIRS) alleles are undergoing global dissemination.

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Figures

Figure
Figure
Pulsed-field gel electrophoresis patterns for Vibrio cholerae isolates from Haiti, 2010–2011.
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