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. 2012 Jan;47(1):93-9.
doi: 10.1007/s11745-011-3627-0. Epub 2011 Nov 20.

Separation of lipid classes by HPLC on a cyanopropyl column

Petter Olsson  1 , Jan Holmbäck, Bengt Herslöf
Affiliations

Separation of lipid classes by HPLC on a cyanopropyl column

Petter Olsson et al. Lipids. 2012 Jan.

Abstract

A new method for the separation and identification of lipid classes by normal-phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non-polar as well as phospholipid classes. The inherent small differences in performances between possible non-polar eluent components of the gradient, such as hexane, heptane, and iso-octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.

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References

    1. J Chromatogr A. 2008 Oct 31;1209(1-2):88-94 - PubMed
    1. J Lipid Res. 1982 Jan;23(1):190-6 - PubMed
    1. Lipids. 2001 Feb;36(2):153-65 - PubMed
    1. J Lipid Res. 1985 Apr;26(4):507-12 - PubMed
    1. Bull Exp Biol Med. 2009 Apr;147(4):489-92 - PubMed

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