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. 2012:809:465-72.
doi: 10.1007/978-1-61779-376-9_30.

Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex

Young-Wook Cho  1 , Sunhwa Hong, Kai Ge
Affiliations

Affinity purification of MLL3/MLL4 histone H3K4 methyltransferase complex

Young-Wook Cho et al. Methods Mol Biol. 2012.

Abstract

Methylation on histone H3 lysine 4 (H3K4) correlates with actively transcribed genes. In mammalian cells, there exist multiple Set1-like histone H3K4 methyltransferase complexes, which have overlapping but distinct subunit compositions. Developing methods to isolate each of these histone H3K4 methyltransferase complexes would help understand the molecular mechanisms by which histone H3K4 methylation regulates mammalian gene expression. In this chapter, we provide a one-step affinity purification protocol on isolation of the MLL3/MLL4 histone H3K4 methyltransferase complex using FLAG-tagged PA1, a unique subunit of the MLL3/MLL4 complex.

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Figures

Fig. 1
Fig. 1
Subunit compositions of the yeast Set1 and human Set1-like histone H3K4 methyltransferase complexes.
See this image and copyright information in PMC

References

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