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. 2012 Feb;50(2):477-9.
doi: 10.1128/JCM.05247-11. Epub 2011 Nov 23.

Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae

Delphine Girlich  1 , Laurent Poirel, Patrice Nordmann
Affiliations

Value of the modified Hodge test for detection of emerging carbapenemases in Enterobacteriaceae

Delphine Girlich et al. J Clin Microbiol. 2012 Feb.

Abstract

The modified Hodge test has an excellent sensitivity for detecting enterobacterial isolates producing Ambler class A (KPC) and class D (OXA-48) carbapenemases. Its sensitivity is low for NDM-1 producers (50%) but is increased to 85.7% by adding ZnSO(4) (100 μg/ml) in the culture medium. However, this test has a low specificity and is time-consuming.

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Figures

Fig 1
Fig 1
MHT on MHA (A) and on MHA added with zinc sulfate (100 μg/ml) (B). Organisms tested: 1, E. coli JM109; 2, K. pneumoniae COO (CTX-M-15 + porin loss); 3, K. pneumoniae BIC (OXA-48); 4, K. pneumoniae POZ (KPC-2); 5, E. coli GEN (NDM-1); 6, E. coli RIC (NDM-1); 7, E. coli ALL (NDM-1). Zinc sulfate improved the MHT for E. coli RIC and not for E. coli ALL.
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References

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