Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;111(4):1519-33.
doi: 10.1083/jcb.111.4.1519.

Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis

R L Bernat  1 , G G Borisy, N F Rothfield, W C Earnshaw
Affiliations

Injection of anticentromere antibodies in interphase disrupts events required for chromosome movement at mitosis

R L Bernat et al. J Cell Biol. 1990 Oct.

Abstract

We have used autoantibodies to probe the function of three human centromere proteins in mitosis. These antibodies recognize three human polypeptides in immunoblots: CENP-A (17 kD), CENP-B (80 kD), and CENP-C (140 kD). Purified anticentromere antibodies (ACA-IgG) disrupt mitosis when introduced into tissue culture cells during interphase. We have identified two execution points for antibody inhibition. Antibodies injected into the nucleus greater than or equal to 3 h before mitosis prevent the chromosomes from undergoing normal prometaphase movements in the subsequent mitosis. Antibodies injected in the nucleus during late G2 cause cells to arrest in metaphase. Surprisingly, antibodies introduced subsequent to the beginning of prophase do not block mitosis. These results suggest that the CENP antigens are involved in two essential interphase events that are required for centromere action in mitosis. These may include centromere assembly coordinate with the replication of alpha-satellite DNA at the end of S phase and the structural maturation of the kinetochore that begins at prophase.

PubMed Disclaimer

References

    1. J Cell Biol. 1987 Jan;104(1):9-18 - PubMed
    1. J Cell Biol. 1984 Apr;98(4):1556-64 - PubMed
    1. J Cell Biol. 1979 Mar;80(3):674-91 - PubMed
    1. J Cell Biol. 1989 Nov;109(5):2245-55 - PubMed
    1. Int Rev Cytol. 1982;79:1-58 - PubMed

Publication types