Prenatal development of retinogeniculate projections in the rabbit: an HRP study

Abstract

The prenatal development of the rabbit's retinal projections to the dorsal lateral geniculate nucleus (dLGN) was studied by using anterograde axonal transport of HRP injected intraocularly. Further, the ontogenesis of the dLGN's alpha and beta sectors was studied. Fetuses aged embryonic day 18 (E18) to E29 were examined. Gestation in the rabbit is 30-31 days. On E18 the future dorsal lateral and medial geniculate nuclei appear as a continuous strip of cells along the lateral margin of the dorsal thalamus. On E21 labelled retinal fibers are invading the lateral margin of the dLGN contralateral, but not ipsilateral, to an injected eye. At this age the dorsal lateral and medial geniculate nuclei are separating. By E23 contralateral fibers occupy the entire presumptive alpha sector, while ipsilateral fibers are invading the caudal half of the sector, overlapping the contralateral fibers. At this age the alpha and beta sectors begin to differentiate. On E25 contralateral fibers are more densely distributed throughout the alpha sector and the ipsilateral fibers are concentrated dorsally within the caudal three-quarters of the sector. By E27 contralateral fibers begin to withdraw from a medial zone of the alpha sector, while ipsilateral fibers remain densest in this zone and begin to withdraw from more lateral and caudal aspects of the sector; contralateral fibers, but not ipsilateral fibers, invade the beta sector. At this age the alpha and beta sectors acquire an adult-like appearance. By E29 the contralateral fibers vacate the beta sector and the medial zone of the dLGN and the ipsilateral fibers are restricted to this zone. Thus, 1 or 2 days before birth, the locations of the ipsilateral and contralateral retinal projections to the dLGN resemble those seen in the adult. The early overlapping projections of ipsilateral and contralateral retinal fibers within the dLGN and their eventual segregation in the fetal rabbit are consistent with the development of these projections in other mammalian orders. Further, the brief invasion of the beta sector by the contralateral fibers resembles the transient occupation of the carnivores' perigeniculate nucleus by developing retinal fibers. In addition, direct comparisons of temporal and spatial events during retinal innervation of the dLGN and the superior colliculus indicate several developmental differences between the two nuclei.