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. 2012 Mar;41(2):186-94.
doi: 10.1097/MPA.0b013e31822862f6.

The proteome of normal pancreatic juice

Courtney J Doyle  1 , Kyle Yancey, Henry A Pitt, Mu Wang, Kerry Bemis, Michele T Yip-Schneider, Stuart T Sherman, Keith D Lillemoe, Michael D Goggins, C Max Schmidt
Affiliations

The proteome of normal pancreatic juice

Courtney J Doyle et al. Pancreas. 2012 Mar.

Abstract

Objectives: The aims of this study were to characterize the proteome of normal pancreatic juice, to analyze the effect of secretin on the normal proteome, and to compare these results with published data from patients with pancreatic cancer.

Methods: Paired pancreatic fluid specimens (before and after intravenous secretin stimulation) were obtained during endoscopic pancreatography from 3 patients without significant pancreatic pathology. Proteins were identified and quantified by mass spectrometry-based protein quantification technology. The human RefSeq (NCBI) database was used to compare the data in samples from patients without pancreatic disease with published data from 3 patients with pancreatic cancer.

Results: A total of 285 proteins were identified in normal pancreatic juice. Ninety had sufficient amino acid sequences identified to characterize the protein with a high level of confidence. All 90 proteins were present before and after secretin administration but with altered relative concentrations, usually by 1 to 2 folds, after stimulation. Comparison with 170 published pancreatic cancer proteins yielded an overlap of only 42 proteins.

Conclusions: Normal pancreatic juice contains multiple proteins related to many biological processes. Secretin alters the concentration but not the spectrum of these proteins. The pancreatic juice proteome of patients without pancreatic disease and that of patients with pancreatic cancer differ markedly.

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Figures

Figure 1
Figure 1. Comparison to Proteome of Pancreatic Adenocarcinoma
Pancreatic juice proteome of normal patients (172 proteins, white) compared with the pancreatic juice proteome of published patients with adenocarcinoma (170 patients, black). 42 proteins (grey) were found in both normal and in adenocarcinoma pancreatic juice.
See this image and copyright information in PMC

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