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. 2011 Jul;18(3):27-32.

Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus)

Syuhaidah Sahabudin  1 , Rosmilah Misnan, Zailatul Hani Mohammad Yadzir, Jamaludin Mohamad, Noormalin Abdullah, Faizal Bakhtiar, Shahnaz Murad
Affiliations

Identification of Major and Minor Allergens of Black Tiger Prawn (Penaeus monodon) and King Prawn (Penaeus latisulcatus)

Syuhaidah Sahabudin et al. Malays J Med Sci. 2011 Jul.

Abstract

Background: Prawns and shrimp are a frequent cause of seafood allergy mediated by IgE antibodies. Penaeus monodon and Penaeus latisulcatus, commonly known as black tiger prawn and king prawn, respectively, are among the most frequently consumed prawns in Malaysia. The aim of this study was to identify the IgE-binding proteins of these 2 prawn species.

Methods: Raw and boiled prawn extracts were prepared and then resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). IgE-immunoblotting was then performed using sera from patients with positive skin prick tests to the raw prawn extracts.

Results: SDS-PAGE analysis of the raw extracts of both prawn species revealed 23 protein bands; the boiled extracts yielded fewer protein bands. The bands in the range of 40 to 100 kDa were sensitive to heat and therefore were not found in the boiled extracts. Immunoblot of raw extracts of black tiger prawns and king prawns yielded 14 and 11 IgE-binding proteins, respectively, with molecular weights of between 15 and 200 kDa. Proteins at 36, 42, and 49 kDa were detected as the major allergens in both species of prawns. A protein of 75 kDa was also identified as a major allergen in black tiger prawns. Other potential allergens were also observed at various molecular masses.

Conclusion: Proteins of 36, 42, and 49 kDa were identified as the major allergens of both species of prawns. The 36 and 42 kDa proteins are hypothesised to be tropomyosin and arginine kinase, respectively. A high molecular weight protein of 75 kDa was found to be an additional major allergen in black tiger prawns.

Keywords: Penaeus; allergens; allergy and clinical immunology; hypersensitivity; immunoblotting; tropomyosin.

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Figures

Figure 1:
Figure 1:
Protein profiles and IgE-binding patterns of raw (A) black tiger prawns and (B) king prawns. Lane STD consists of molecular weight markers (in kDa). Lanes UC and C contain protein profiles of raw and boiled extracts, respectively. Lanes B and N contain blank and negative controls, respectively. Lanes 1 to 22 contain the immunoblotting results of different serum samples.
Figure 1:
Figure 1:
Protein profiles and IgE-binding patterns of raw (A) black tiger prawns and (B) king prawns. Lane STD consists of molecular weight markers (in kDa). Lanes UC and C contain protein profiles of raw and boiled extracts, respectively. Lanes B and N contain blank and negative controls, respectively. Lanes 1 to 22 contain the immunoblotting results of different serum samples.
Figure 2:
Figure 2:
The frequency of specific IgE-binding of sera from patients with raw (A) black tiger prawn allergy and (B) king prawn allergy by immunoblotting analysis.
Figure 2:
Figure 2:
The frequency of specific IgE-binding of sera from patients with raw (A) black tiger prawn allergy and (B) king prawn allergy by immunoblotting analysis.
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