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. 1978:(24 Pt 1):97-103.

Characterization of human cytomegalovirus DNA: infectivity and molecular weight

  • PMID: 221366

Characterization of human cytomegalovirus DNA: infectivity and molecular weight

J L Geelen et al. IARC Sci Publ (1971). 1978.

Abstract

Human cytomegalovirus DNA was isolated from purified virions and further purified by sucrose density-gradient centrifugation. The viral DNA molecules were studied by electron microscopy and found to be linear and to have a length of 76.22 +/- 3.80 microM, corresponding to a molecular weight of 147.13 +/- 6.18 x 10(6). The DNA was infectious when tested in human embryonic lung cells using the DEAE-dextran and the calcium-phosphate techniques. The density in caesium chloride was 1.717 g/cm3.

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