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. 2012 Jan;40(Database issue):D809-14.
doi: 10.1093/nar/gkr1170. Epub 2011 Dec 1.

MMMDB: Mouse Multiple Tissue Metabolome Database

Masahiro Sugimoto  1 , Satsuki Ikeda, Kanako Niigata, Masaru Tomita, Hideyo Sato, Tomoyoshi Soga
Affiliations

MMMDB: Mouse Multiple Tissue Metabolome Database

Masahiro Sugimoto et al. Nucleic Acids Res. 2012 Jan.

Abstract

The Mouse Multiple Tissue Metabolome Database (MMMDB) provides comprehensive and quantitative metabolomic information for multiple tissues from single mice. Manually curated databases that integrate literature-based individual metabolite information have been available so far. However, data sets on the absolute concentration of a single metabolite integrated from multiple resources are often difficult to be used when different metabolomic studies are compared because the relative balance of the multiple metabolite concentrations in the metabolic pathways as a snapshot of a dynamic system is more important than the absolute concentration of a single metabolite. We developed MMMDB by performing non-targeted analyses of cerebra, cerebella, thymus, spleen, lung, liver, kidney, heart, pancreas, testis and plasma using capillary electrophoresis time-of-flight mass spectrometry and detected 428 non-redundant features from which 219 metabolites were successfully identified. Quantified concentrations of the individual metabolites and the corresponding processed raw data; for example, the electropherograms and mass spectra with their annotations, such as isotope and fragment information, are stored in the database. MMMDB is designed to normalize users' data, which can be submitted online and used to visualize overlaid electropherograms. Thus, MMMDB allows newly measured data to be compared with the other data in the database. MMMDB is available at: http://mmmdb.iab.keio.ac.jp.

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Figures

Figure 1.
Figure 1.
An MMMDB screenshot. (A) List of metabolites that fulfilled the search condition. (B) List of the registered entries of the metabolites selected from panel (A). (C) Overlaid electropherograms displayed by the Multi Graph option. The other options, Electropherogram and Mass Spectrum, will display a single electropherogram or the mass spectrum, respectively. (D) The search conditions used. (E) The molecular structure of the displayed metabolite. (F) Tissue graph showing the quantified concentrations of the selected metabolites in each tissue.
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