Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Apr;124(6):1005-15.
doi: 10.1007/s00122-011-1764-8. Epub 2011 Dec 6.

Two loci in sorghum with NB-LRR encoding genes confer resistance to Colletotrichum sublineolum

Affiliations

Two loci in sorghum with NB-LRR encoding genes confer resistance to Colletotrichum sublineolum

Moses Biruma et al. Theor Appl Genet. 2012 Apr.

Abstract

The aim of this work was to identify plant resistance genes to the sorghum anthracnose fungus Colletotrichum sublineolum. cDNA-AFLP transcript profiling on two contrasting sorghum genotypes inoculated with C. sublineolum generated about 3,000 informative fragments. In a final set of 126 sequenced genes, 15 were identified as biotic stress related. Seven of the plant-derived genes were selected for functional analysis using a Brome mosaic virus-based virus-induced gene silencing (VIGS) system followed by fungal inoculation and quantitative real-time PCR analysis. The candidate set comprised genes encoding resistance proteins (Cs1A, Cs2A), a lipid transfer protein (SbLTP1), a zinc finger-like transcription factor (SbZnTF1), a rice defensin-like homolog (SbDEFL1), a cell death related protein (SbCDL1), and an unknown gene harboring a casein kinase 2-like domain (SbCK2). Our results demonstrate that down-regulation of Cs1A, Cs2A, SbLTP1, SbZnF1 and SbCD1 via VIGS, significantly compromised the resistance response while milder effects were observed with SbDEFL1 and SbCK2. Expanded genome analysis revealed that Cs1A and Cs2A genes are located in two different loci on chromosome 9 closely linked with duplicated genes Cs1B and Cs2B, respectively. The nucleotide binding-leucine rich repeat (NB-LRR) encoding Cs gene sequence information is presently employed in regional breeding programs.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Plant Physiol. 2005 May;138(1):393-401 - PubMed
    1. Genetics. 2010 Apr;184(4):915-26 - PubMed
    1. Plant J. 2000 Jan;21(2):177-88 - PubMed
    1. Cell Microbiol. 2009 Oct;11(10):1453-61 - PubMed
    1. Plant J. 2009 Oct;60(2):218-26 - PubMed

Publication types

LinkOut - more resources