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. 2011 Dec;26(12):1556-62.
doi: 10.3346/jkms.2011.26.12.1556. Epub 2011 Nov 29.

Prognostic value of immunohistochemical biomarkers at different cut-off values in patients with diffuse large B-cell lymphoma treated with CHOP chemotherapy

Sukjoong Oh  1 , Dong Hoe Koo, Cheolwon Suh, Shin Kim, Bong Hee Park, Joon Kang, Jooryung Huh
Affiliations

Prognostic value of immunohistochemical biomarkers at different cut-off values in patients with diffuse large B-cell lymphoma treated with CHOP chemotherapy

Sukjoong Oh et al. J Korean Med Sci. 2011 Dec.

Abstract

Many predictive models have been proposed for better stratification of diffuse large B-cell lymphoma (DLBCL). Hans' algorithm has been widely used as standard to sub-classify DLBCL into germinal center B-cell (GCB) and non-GCB origins. However, there have been disagreements in the literature regarding its prognostic significance. Here, we retrospectively analyzed Hans' algorithm and the individual immunohistochemical biomarkers at different cut-off values (5%, 30%, 50% or 75%) in 94 Korean patients with DLBCL treated with combination chemotherapy with cyclophosphamide, daunorubicin, vincristine, and prednisone. No significant differences were observed between the GCB (18 patients, 19.1%) and non-GCB (76, 80.9%) groups. Among individual biomarkers, CD10 negativity (cut point: 30%) and bcl-6 positivity (cut point: 5%) were independent good prognostic markers in progression-free survival (PFS), whereas bcl-6 (cut point: 5%) positivity was an independent good prognostic marker in overall survival irrelevant of international prognostic index. The present study showed the lack of predictability of Hans' algorithm in DLBCL patients, and that CD10, Bcl-6 may have diverse prognostic significance at different cut-off values. Our results suggest that the proposed cut-off value may not be applied universally, and that the optimal cut-off value may need to be optimized for individual laboratory.

Keywords: Bcl-6; CD10; CHOP Chemotherapy; Diffuse Large B-cell Lymphoma (DLBCL); Germinal Center B-cell (GCB); Hans' Algorithm; Non-germinal Center B-cell (Non-GCB).

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Figures

Fig. 1
Fig. 1
Kaplan-Meier curves of progression-free survival (PFS) (A) and overall survival (OS) (B) based on stratifying diffuse large B cell lymphomas (DLBCL) into germinal center B-cell (GCB) and non-germinal center B-cell (non-GCB) groups.
Fig. 2
Fig. 2
Kaplan-Meier curves of progression free survival (PFS) according to CD10 (cut-off 30%) (A) and bcl-6 (cut-off 5%) (B).
Fig. 3
Fig. 3
Kaplan-Meier curves of overall survival (OS) according to bcl-6 (cut-off 5%).
See this image and copyright information in PMC

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