doi: 10.1093/nar/18.19.5729.
Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine
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- PMID: 2216766
- PMCID: PMC332307
- DOI: 10.1093/nar/18.19.5729
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Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine
Nucleic Acids Res.
.
Free PMC article
Abstract
A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethylthymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphitetriester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed.
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