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. 1990 Oct 11;18(19):5729-34.
doi: 10.1093/nar/18.19.5729.

Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine

D Fernandez-Forner  1 , Y Palom, S Ikuta, E Pedroso, R Eritja
Affiliations
Free PMC article

Synthesis and characterization of oligodeoxynucleotides containing the mutagenic base analogue 4-O-ethylthymine

D Fernandez-Forner et al. Nucleic Acids Res. .
Free PMC article

Abstract

A method for the preparation of oligonucleotides containing the mutagenic base 4-O-ethylthymine is described for the first time. Use of p-nitrophenylethyl type base protecting groups together with phosphitetriester solid-phase methodology makes possible the rapid and efficient preparation of oligonucleotides bearing 4-O-ethylthymine, while standard base protecting groups are not compatible with the presence of this base. Possible applications of this methodology are discussed.

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References

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