doi: 10.1111/j.1365-2818.2011.03577.x.
Epub 2011 Dec 15.
Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam
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- PMID: 22171566
- DOI: 10.1111/j.1365-2818.2011.03577.x
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Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam
J Microsc.
2012 Mar.
Abstract
In this paper we report stimulated emission depletion (STED) and two-photon excitation (2PE) fluorescence microscopy with continuous wave (CW) laser beam using a new generation laser scanning confocal microscope equipped for STED-CW (TCS STED-CW, Leica Microsystems, Mannheim, Germany). We show the possibility to achieve CW-2PE with the very same beam used for STED-CW. This feature extends the performance of the microscope allowing multimodal imaging (CW-2PE, STED-CW, confocal).
© 2011 The Authors Journal of Microscopy © 2011 Royal Microscopical Society.
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