Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;87(19):7467-71.
doi: 10.1073/pnas.87.19.7467.

Increased expression of a 58-kDa protein kinase leads to changes in the CHO cell cycle

B A Bunnell  1 , L S Heath, D E Adams, J M Lahti, V J Kidd
Affiliations

Increased expression of a 58-kDa protein kinase leads to changes in the CHO cell cycle

B A Bunnell et al. Proc Natl Acad Sci U S A. 1990 Oct.

Erratum in

  • Proc Natl Acad Sci U S A 1991 Mar 15;88(6):2612

Abstract

We have isolated and characterized cDNA encoding a human 58-kDa protein kinase that is homologous to the cell division control (CDC) protein kinases. This protein kinase also contains a unique N-terminal domain that may potentially regulate its function. Due to its relatedness to p34CDC2, the human p58 cDNA was overexpressed in CHO cells to determine the effect on the cell cycle. Elevated expression of p58 in these cells resulted in prolonged late telophase and early G1 phase of the cell cycle. These p58 overexpressors showed a significantly increased frequency of tubulin midbodies as well as significant increases in mitotic abnormalities. Thus, proper regulation of p58 protein kinase is essential for normal cell cycle progression in these cells.

PubMed Disclaimer

References

    1. Science. 1975 Mar 14;187(4180):948-50 - PubMed
    1. Science. 1988 Jul 1;241(4861):42-52 - PubMed
    1. Methods Enzymol. 1983;100:468-500 - PubMed
    1. Nature. 1988 Nov 17;336(6196):251-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2351-5 - PubMed

Publication types

MeSH terms

Associated data