Genogroup I and II picobirnaviruses in respiratory tracts of pigs

Abstract

Sequence-independent amplification and specific reverse transcription PCRs identified genogroup I and II picobirnaviruses in respiratory tracts of pigs. These data expand knowledge of picobirnavirus diversity and tropism. Genetic relationships between porcine respiratory and human enteric picobirnaviruses suggest cross-species transmission of picobirnaviruses between pigs and humans.

Figure A1

Neighbor-joining (Jukes-Cantor model) phylogenetic tree of an ≈165-bp fragment of the genogroup I picobirnavirus RNA-dependent RNA polymerase gene from known human, porcine, and wastewater genogroup I picobirnaviruses and newly characterized porcine respiratory genogroup I picobirnaviruses (sequences are available on request). Each branch represents a sequence or group of sequences (95% identical with gaps) depending upon the presence of a colored block. Every branch corresponds to the adjacent information, which indicates source, year of isolation, and location of virus variants. Reference sequences from published studies are identified by their GenBank accession numbers. For species, H, human; WW, wastewater; S, snake; D, dog; P; pig; R, rat. For countries of isolation, USA, United States; NL, the Netherlands; Br, Brazil; H, Hungary; A, Argentina; T, Thailand; In, India; V, Venezuela; Ch, People’s Republic of China. Sequences from newly identified porcine respiratory picobirnaviruses in this study are shown according to recent nomenclature proposals (7). Isolates in red are branches in which porcine respiratory picobirnaviruses are present. Scale bar indicates nucleotide substitutions per site.

Figure

Phylogenetic analysis of genogroup II picobirnaviruses. Neighbor-joining (Jukes-Cantor model) phylogenetic tree of an ≈339-bp fragment (reference strain 4-GA-91 (9) of the picobirnavirus genogroup II RNA-dependent RNA polymerase gene from known human picobirnaviruses and newly characterized porcine picobirnaviruses in this study (JN176312–315, shown in red). Significant bootstrap values are shown. Nomenclature of depicted viruses is according to recent proposals (7). PBVII/Homo sapiens/USA-4-GA-91/1991 (AF246940) (9); PBVII/Homo sapiens/NLD-VS142–3/2007 (GU968925) (6); and PBVII/Homo sapiens/IND-GPBV6G2P/2007 (AB526257). Scale bar indicates nucleotide substitutions per site.