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. 2012 Jan 27;51(5):1169-72.
doi: 10.1002/anie.201106313. Epub 2011 Dec 15.

Immunopods: polymer shells with native antibody cross-links

Ke Zhang  1 , Dan Zheng, Liangliang Hao, Joshua I Cutler, Evelyn Auyeung, Chad A Mirkin
Affiliations

Immunopods: polymer shells with native antibody cross-links

Ke Zhang et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
Synthesis of protein-conjugated hollow polymer nanopods (R=Br or -NHCH2CH2NHCOCH2CH2OCH2CCH).
Figure 2
Figure 2
A) A two-protein reporter assay designed to evaluate the successful formation of protein-nanopod conjugates. B) The blue color indicates that HRP-modified particles are immobilized on the biotinylated surface after extensive washing (except bottom row). Lanes 1–3: samples containing SA, HRP, and polymer 1; lanes 4 and 5: control samples lacking either SA or polymer 1. Top row: AuNP–protein conjugates; middle row: protein–nanopod conjugates; bottom row: protein–nanopod conjugates directly combined with 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 developing solution as a control to determine if HRP remains active after dissolution of the gold core. This assay indicates that protein–nanopod conjugates containing both HRP and SA are successfully formed.
Figure 3
Figure 3
A) TEM images of a) ca. 10 nm AuNPs and b) IPs synthesized from ca. 10 nm AuNPs (negatively stained using uranyl acetate). B) SDS-PAGE of IPs and controls under a) native and b) denaturing conditions. The antibody and the IP can be observed by white light while the polymer component alone cannot be observed. By fluorescence in this experiment, the polymer and IP can be observed but not the antibody, which was not labeled.
Figure 4
Figure 4
A) Confocal microscopy images of MCF-7 cells treated with 10 nm anti-HER2 IPs (top row) and a control mixture containing equivalent concentrations (10 nm) of free antibody and polymer (bottom row). While antibody binding is observed for both IPs and the control mixture, polymer association with cells is only significant when the IPs are used. B) Flow cytometry analysis of IP binding. Left: antibody (NL637) channel. Right: polymer nanopod (fluorescein) channel.
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