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. 2012 Mar;50(3-4):701-6.
doi: 10.1016/j.fct.2011.11.055. Epub 2011 Dec 9.

Vitamin E restriction in the diet enhances phagocytic activation by dichloroacetate and trichloroacetate in mice

Ezdihar A Hassoun  1 , Ali Al-Dieri
Affiliations

Vitamin E restriction in the diet enhances phagocytic activation by dichloroacetate and trichloroacetate in mice

Ezdihar A Hassoun et al. Food Chem Toxicol. 2012 Mar.

Abstract

The effects of a vitamin E-restricted diet on the induction of phagocytic activation by dichloroacetate (DCA) and trichloroacetate (TCA) was investigated. Groups of B6C3F1 male mice were either kept on standard diet (Std diet group) or diet that had the vitamin provided only by its natural ingredients (Low-E diet group). The animals in each diet group were administered 77 mg of DCA or TCA/ kg/day, or 5 ml/kg water (controls), by gavage, for 13 weeks. Thereafter, peritoneal lavage cells (PLC) were assayed for superoxide anion (SA), tumor necrosis factor (TNF)-α, and myeloperoxidase (MPO), as well as for the activities of the anti-oxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). SA and TNFα production, as well as MPO, SOD, CAT and GSH-Px activities were significantly increased in the cells from the Low-E diet group treated with the compounds as compared with cells from hosts in the Std-diet group that received the corresponding treatments. The results indicate that consumption of a Vitamin E-restricted diet enhances the induction of phagocytic activation by DCA and TCA, a mechanism that was previously suggested to be an initial adaptive/protective response against the compounds long-term effects.

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Figures

Figure 1
Figure 1. SA production in PLC of mice maintained on Std or Low-E diet, and treated subchronically with DCA or TCA
Values shown are in terms of cytochrome c reduced/min/mg protein present. Columns that are labeled with non-identical superscript are significantly different (p < 0.05).
Figure 2
Figure 2. MPO activity in PLC of mice maintained on Std or Low-E diet, and treated sub-chronically with DCA or TCA
Values are in terms of units/mg protein present at 25°C. Columns that are labeled with non-identical superscript are significantly different (p < 0.05).
Figure 3
Figure 3. TNFα production in PLC of mice maintained on Std or Low-E diet, and treated sub-chronically with DCA or TCA
Values shown are in terms of pg TNFα/mg protein present Columns that are labeled with non-identical superscript are significantly different (p < 0.05).
Figure 4
Figure 4. SOD activity in PLC of mice maintained on Std or Low-E diet, and treated sub-chronically with DCA or TCA
Values are in terms of units/mg protein. Columns that are labeled with non-identical superscript are significantly different (p < 0.05).
Figure 5
Figure 5. GSH-Px activity in PLC of mice maintained on Std or Low-E diet, and treated sub-chronically with DCA or TCA
Values are in terms of units/mg protein. Columns that are labeled with non-identical super-script are significantly different (p < 0.05).
Figure 6
Figure 6. CAT activity in PLC of mice maintained on Std or Low-E diet, and treated sub-chronically with DCA or TCA
Values are in terms of units/mg protein. Columns that are labeled with non-identical superscript are significantly different (p < 0.05).
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References

    1. Babior BM, Kipnes RS, Curnutte JT. Biological defense mechanism. The production by leukocytes of superoxide, a potential bactericidal agent. J. Clin. Invest. 1973;52:741–744. - PMC - PubMed
    1. Bensoussan K, Morales CR, Hermo L. Vitamin E deficiency causes incomplete spermatogenesis and affects the structural differentiation of epithelial cells of the epidydymis in the rat. J. Androl. 1998;19:266–288. - PubMed
    1. Boxer L. Regulation of phagocyte function by α-tocopherol. Proc. Nutr. Soc. 1986;45:333–344. - PubMed
    1. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J. Invest. Dermatol. 1982;78:206–209. - PubMed
    1. Bull RJ, Sanchez IM, Nelson MA, Larson JL, Lansing AJ. Liver tumor induction in B6C3F1 mice by dichloroacetate and dichloroacetate. Toxicology. 1990;63:341–359. - PubMed

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