Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Editorial
. 2012 Jun;235(2):397-401.
doi: 10.1016/j.expneurol.2011.12.004. Epub 2011 Dec 8.

Studying microRNAs in the brain: technical lessons learned from the first ten years

Sébastien S Hébert, Peter T Nelson
Editorial

Studying microRNAs in the brain: technical lessons learned from the first ten years

Sébastien S Hébert et al. Exp Neurol. 2012 Jun.
No abstract available

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
miRNA deep sequencing of the human brain. RNA isolated from the superior and middle temporal gyrus was analyzed using deep sequencing (see text for more details), resulting in slightly over 1,000,000 reads of annotated miRNAs. Note the Y axis uses a logarithmic scale to describe the number of a particular miRNA species; each blue diamond is a particular annotated miRNA. Fewer than 900 total annotated miRNAs were detected, of which fewer than 200 were detected with more than 100 reads in this sample. More than one-third (358,073) of the total miRNAs were represented in the top four high copy number miRNAs. These data underscore the point that relatively few miRNAs are highly expressed in brain.
Fig. 2
Fig. 2
3′UTR length heterogeneity as observed using 3′RACE-PCR. RNA was isolated from wildtype mouse organs, mouse primary cortical neurons, and human MCF-7 cells were used to perform 3′ Rapid Amplification of cDNA 3′ Ends (RACE) PCR using APP-specific oligonucleotides. These assays reveal changes in APP 3′UTR length and abundance depending on developmental stage, age, cell type and organ. Arrows indicate various 3′UTR products. Different 3′UTRs for a given transcript is probably one of many ways that miRNA targeting is regulated in the brain.
See this image and copyright information in PMC

References

    1. Adams BD, Claffey KP, White BA. Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 2009;150:14–23. - PMC - PubMed
    1. Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136:215–233. - PMC - PubMed
    1. Carter MG, Sharov AA, VanBuren V, Dudekula DB, Carmack CE, Nelson C, Ko MS. Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray. Genome Biol. 2005;6:R61. - PMC - PubMed
    1. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res. 2005;33:e179. - PMC - PubMed
    1. Chi SW, Zang JB, Mele A, Darnell RB. Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps. Nature. 2009;460:479–486. - PMC - PubMed

Publication types