Complete genome sequence of Ferroglobus placidus AEDII12DO

Abstract

Ferroglobus placidus belongs to the order Archaeoglobales within the archaeal phylum Euryarchaeota. Strain AEDII12DO is the type strain of the species and was isolated from a shallow marine hydrothermal system at Vulcano, Italy. It is a hyperthermophilic, anaerobic chemolithoautotroph, but it can also use a variety of aromatic compounds as electron donors. Here we describe the features of this organism together with the complete genome sequence and annotation. The 2,196,266 bp genome with its 2,567 protein-coding and 55 RNA genes was sequenced as part of a DOE Joint Genome Institute Laboratory Sequencing Program (LSP) project.

Keywords: Archaea; Archaeoglobales; Euryarchaeota; anaerobe; hydrothermal vent; hyperthermophile.

Figure 1

16S ribosomal RNA phylogenetic tree of Archaeoglobaceae. The tree was generated with weighbor [9] through the Ribosomal Database Project website [10] and displayed with njplot [11]. Organisms with two asterisks after the name are those with complete genomes sequenced and published. Those with one asterisk have a genome project in progress, according to the Genomes OnLine Database [12]. Methanocaldococcus jannaschii is the outgroup.

Figure 2

Graphical circular map of the chromosome. From outside to the center: Genes on forward strand (colored by COG categories), genes on reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, and GC skew.

Figure 3

Central metabolism of the hyperthermophilic archaeon Ferroglobus placidus. Abbreviations: G6PI: glucose-6-phosphate isomerase. FBPase: fructose-1,6-bisphosphatase. FBPA: fructose-1,6-bisphosphate aldolase. G3PDH: glycerol-3-phosphate dehydrogenase. TPI: triose-phosphate isomerase. PGK: phosphoglycerate kinase. PGM: phosphoglycerate mutase. PEP: phosphoenolpyruvate. PEPC: phosphoenolpyruvate carboxylase. PK: pyruvate kinase. PPDK: phosphoenolpyruvate dikinase. OadC: oxaloacetate decarboxylase. POR: pyruvate ferredoxin reductase (pyruvate synthase). ACL: acetyl-CoA ligase. AP: acetate phosphatase. CS: citrate synthase. ACN: aconitase. ICDH: isocitrate dehydrogenase. AKGD: alpha-ketoglutarate dehydrogenase. SuCoAS: succinyl-CoA synthase. SDH: succinate dehydrogenase. FUM: fumarase. MDH: malate dehydrogenase. PHI: 3-hexulose-6-phosphate isomerase. HPS: 3-hexulose-6-phosphate synthase. RPI: ribose-5-phosphate isomerase. FDH: formate dehydrogenase. MF: methanofuran. FMFDH: formylmethanofuran dehydrogenase. THM: tetrahydromethanopterin. THMFT: tetrahydromethanopterin formyl transferase. MTHMC: methenyltetrahydromethanopterin cyclohydrolase. MTHMR: methylentetrahydromethanopterin reductase. CODH/ACS: CO dehydrogenase/acetyl-CoA synthase. Gene designations in colors indicates association in clusters.