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. 2012:723:799-805.
doi: 10.1007/978-1-4614-0631-0_102.

Identification of pigment epithelium-derived factor receptor (PEDF-R) antibody epitopes

Preeti Subramanian  1 , Matthew Rapp, S Patricia Becerra
Affiliations

Identification of pigment epithelium-derived factor receptor (PEDF-R) antibody epitopes

Preeti Subramanian et al. Adv Exp Med Biol. 2012.
No abstract available

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Figures

Fig. 102.1
Fig. 102.1
Western blot of recombinant PEDF-R polypeptides. Full-length PEDF-R and C-terminal truncated PEDF-R4 were expressed using in vitro cell-free Escherichia coli expression system. Purified proteins were resolved by SDS-PAGE and electrotransfered to a membrane for immunostaining. Photographs of blots immunostained with anti-Xpress (a) and anti-PEDF-R (b) are shown. Lanes 1 and 3 were PEDF-R, lanes 2 and 4 were PEDF-R4. Migration positions of PEDF-R and PEDF-R4 are indicated with arrows, and of molecular weight markers are in between the two blots
Fig. 102.2
Fig. 102.2
Schematic of rat PEDF-R transcript. Transcript summary information for rat PEDF-R was obtained from http://www.ensembl.org for ENSRNOT00000025319. Exons are illustrated by boxes; coding regions are black and gray; introns are the lines flanking the boxes. Expanded region illustrates the design of synthetic peptides
Fig. 102.3
Fig. 102.3
Slot blot of PEDF-R peptides. Peptides (1 mg) were applied to a nitrocellulose membrane using a slot-blot technique and immunostained with anti-PEDF-R. Peptides are indicated to the top of the photograph
Fig. 102.4
Fig. 102.4
Western blot of native PEDF-R from retina R28 cells. Membrane fractions obtained from R28 cells were resolved by SDS-PAGE. Total protein loaded in lanes 1–4 was 6 μg each. Lanes 1 and 2, and lanes 3 and 4 were replicates. Immunoreactions with anti-PEDF-R were for lanes 1 and 2, and with anti-PEDF-R preincubated with peptides E4a and E4b were for lanes 3 and 4. Migration positions of PEDF-R isoforms are indicated with arrows, and molecular weight markers are in the center
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