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Comment
. 2011 Dec 27;108(52):20859-60.
doi: 10.1073/pnas.1118670109. Epub 2011 Dec 19.

Homologous recombination in Nannochloropsis: a powerful tool in an industrially relevant alga

Donald P Weeks  1
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Comment

Homologous recombination in Nannochloropsis: a powerful tool in an industrially relevant alga

Donald P Weeks. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Targeted gene replacement of the NR gene in Nannochloropsis sp. (strain W2J3B). Transformation of Nannochloropsis cells containing a WT copy of the NR gene with a DNA fragment containing the zeocin resistance gene flanked by ∼1-kbp sections of the NR gene results in highly efficient replacement of the native NR gene by homologous recombination and the creation of zeocin resistant, NR-deficient (knockout) mutants. The molecular tools developed by Kilian et al. (1) and other factors (listed) make Nannochloropsis sp. (strain W2J3B) attractive both as a model algal system and as an organism for commercial production of algal biofuels and other high-value biomolecules.
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References

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